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1.17.98.3
formate + oxidized coenzyme F420 = CO2 + reduced coenzyme F420
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1.17.98.3
formate + oxidized coenzyme F420 = CO2 + reduced coenzyme F420
the enzyme is specific for the si face hydride transfer to C5 of coenzyme F420. While catalysis probably occurs by hydride transfer from formate to the enzyme to generate an enzyme-H2 species and then by hydride transfer back out to F420, the formate-derived hydrogen exchanges with solvent protons before transfer back out to coenzyme F420. The kinetics of hydride transfer from formate reveals that this step is not rate determining
726924
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