EC Number |
Reaction |
Reference |
---|
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase |
440116, 440118, 440123, 440125 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
amino acid composition and terminal sequence determination |
440121 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
amino acid sequence determination |
440125 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
cytochrome P-450 enzyme |
440127, 440128, 440130, 440131, 440132 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
cytochrome P-450 type is specific for hydroxylation site of 25-hydroxyvitamin D3 |
440114 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
enzyme can be reconstituted with the 3 components: cytochrome P-450D1alpha, NADPH-renodoxin reductase and renodoxin |
440115 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
ferredoxin, ferredoxin reductase and cytochrome P-450 |
285537, 440111, 440113, 440114, 440118 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
kidney mitochondrial 25-hydroxyvitamin D3-1alpha-hydroxylation system consists of a renal ferredoxin reductase (flavoprotein), renal ferredoxin and cytochrome P-450 |
440113 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase |
440112, 440113, 440124, 440125, 440126, 440127, 440131 |
1.14.15.18 | calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O |
reconstitution of the enzyme system in E. coli cells, consisting of NADH-adrenodoxin reductase, adrenodoxin and CYP27B1 |
440131 |