EC Number   |
Reaction |
Reference |
|---|
    1.1.3.15 | an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 |
A flavoprotein, FMN. Exists as two major isoenzymes. The A form preferentially oxidizes short-chain aliphatic hydroxy acids, and was previously listed as EC 1.1.3.1, glycolate oxidase. The B form preferentially oxidizes long-chain and aromatic hydroxy acids. The rat isoenzyme B also acts as L-amino acid oxidase, EC 1.4.3.2 |
- |
| 1.1.3.15 | an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 |
reaction mechanism, quantum mechanics/molecular mechanics calculations and molecular dynamics simulations, overview. LCHAO-catalyzed dehydrogenation of L-lactate is a stepwise catalytic reaction in a hydride transfer mechanism but not a carbanion mechanism. In contrast to the wild-type enzyme, the mutant Y129F LCHAO catalyzes the oxidation of L-lactate in one step |
745609 |
| 1.1.3.15 | an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 |
the catalytic residue is Phe23, reaction mechanism |
671994 |
| 1.1.3.15 | an (S)-2-hydroxy carboxylate + O2 = a 2-oxo carboxylate + H2O2 |
the pH affects the kinetic steps of the catalytic mechanism of human glycolate oxidase, the enzyme shows a ping-pong bi-bi kinetic mechanism between pH 6.0 and 10.0, overview. Formation of the enzyme-substrate complex suggests the presence of a protonated group participating in substrate binding |
698951 |
