EC Number   |
Reaction |
Reference |
|---|
   6.5.1.2 | NAD+ + [DNA ligase]-L-lysine = [DNA ligase]-N6-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide |
(1a) |
- |
| 6.5.1.2 | [DNA ligase]-N6-(5'-adenylyl)-L-lysine + 5'-phospho-(deoxyribonucleotide)m = 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m + [DNA ligase]-L-lysine |
(1b) |
- |
| 6.5.1.2 | (deoxyribonucleotide)n-3'-hydroxyl + 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP |
(1c) |
- |
| 6.5.1.2 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide |
3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond |
1979, 649250, 649444, 650937, 650967, 651073, 651212, 651683, 652175, 652360, 652463, 652489, 652786, 653376, 653379, 653388, 653923 |
| 6.5.1.2 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide |
mechanism, the initial step is most likely a nucleophilic attack of the epsilon-amino group of a Lys on the adenylyl phosphorus of NAD+ |
1909, 1974 |
| 6.5.1.2 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide |
overall reaction |
- |
| 6.5.1.2 | ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide |
the second step is the transfer of the AMP moiety from the enzyme to the 5'-phosphoryl of the DNA substrate, recreating a diphosphate linkage and thereby preserving the high-energy bond. The third step is the nucleophilic attack of the adjacent 3'-hydroxyl group to form a phosphodiester bond and eliminate AMP |
1974 |
