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Enzyme, Ligand
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4.1.2.10
(R)-mandelonitrile = cyanide + benzaldehyde
based on the binding geometry, a reaction mechanism is proposed that involves one of the two conserved active site histidine residues acting as a general base abstracting the proton from the cyanohydrin hydroxyl group. Site-directed mutagenesis shows that both active site histidines are required for the reaction to occur
702292
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