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Results 1 - 7 of 7
EC Number
Reaction
Commentary
Reference
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
hydrolysis of the phosphodiester bonds of single-stranded RNA at the 3'-side of guanosine nucleotides occurs with high specificity
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
mechanism
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
small single-domain protein of 104 amino acid residues that cleaves single-stranded RNA specifically at the 3'-side of guanine residues in a two-step mechanism
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
the conjugate base of the gamma-carboxyl group of Clu46 is required for tight binding of guanine and hypoxanthine moieties at the primary recognition site of the enzyme
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
the enzyme is composed of 104 amino acid residues and it cleaves single-stranded RNA specifically after guanosine residues to produce mono or oligo-ribonucleotides with terminal 3'-phosphate
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
the reaction is a transphosphorylation yielding a 2',3'-cyclophosphate. This cyclic intermediate is hydrolyzed by a water molecule to a 3'-phosphate in a separate step which is the reversal of the transphosphorylation. The transphosphorylation consists of a nucleophilic in-line inversion displacement at the phosphorous atom of the 5'-leaving group by the entering 2'-oxygen. Tyr38, His40, Glu58, His92 and Phe100 are involved in the catalysis of RNA degradation. During cyclization, Glu58 and His92 serve as the catalytic base and acid, respectively
two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Gp with 2',3'-cyclic phosphate intermediates
the side chain carboxyl of Glu54 accepts a proton from the ribose 2'OH group and the protonated imidazole ring of His85 donates a proton to the leaving 5'O group. The positively charged side chain of Arg65 is to promote the formation of a negatively charged, pentacovalent intermediate state of the phosphate group
Results 1 - 7 of 7