EC Number |
Reaction |
Reference |
---|
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
ordered bi-bi mechanism, NAD+ is the first substrate and NADH is the last product |
287875 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
ordered kinetic mechanism with NAD+ adding before formate |
288085 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
sequential mechanism |
288088 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
sequential ordered bi-bi mechanism |
288101 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
ordered mechanism |
288113 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
hydride-transfer step is rate-limiting |
669327 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
ordered BiBi mechanism with NAD+ and NADH being the first substrate and the second product, resp. |
673381 |
1.17.1.9 | formate + NAD+ = CO2 + NADH + H+ |
reaction mechanism: step I, formate binds directly to Mo, displacing Se-Cys140. Step II, the alpha-proton from formate may be transferred to the nearby His141 that acts as general base. Alternatively, step II may involve a selenium-carboxylated intermediate. Step III, electrons from MoIV are transferred via the 4Fe-4S center to an external electron acceptor and the catalytic cycle is completed |
674932 |