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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 7.5.2.5active recombinant His8-tagged wild-type and mutant LptB enzymes and His6-tagged LptB-LptFG from Escherichia coli strains for activity assays and crystallization are purified by ultracentrifugation, nickel affinity chromatography, ultrafiltration, and gel filtration, recombinant His6/8-tagged enzyme for immunoblotting is purified from Escherichia coli by ultracentrifugation, nickel affinity chromatography, trichloroacetate precipitation and acetone wash, boiling, and SDS-PAGE 752031
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