EC Number   |
|---|
   7.4.2.6 | - |
| 7.4.2.6 | OppA without lipid anchor is produced in the cytoplasm of Lactobacillus lactis where endogenous peptides are available for binding. These peptides bind so tightly that they remain associated with the protein throughout the purification, they can be removed from the protein only by guanidium chloride treatment generating ligand-free OppA |
| 7.4.2.6 | recombinant His-tagged wild-type and mutant enzymes from Lactococcus lactis by nickel affinity chromatography |
| 7.4.2.6 | recombinant refolded and soluble His-tagged OppA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
| 7.4.2.6 | recombinant selenium-labeled maltose-binding fusion protein by amylose affinity chromatography, cleavage of the fusion protein by factor Xa, further purification by ion exchange chromatography and gel filtration, to homogeneity |
| 7.4.2.6 | Sepharose column chromatography, and Superdex 200 gel filtration |
| 7.4.2.6 | TALON metal affinity resin column chromatography |
