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Search Purification (Commentary)

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Results 1 - 10 of 17 > >>
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1-
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1DEAE-Sepharose column chromatography and hydroxyapatite column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1detergent-solubilized transhydrogenase, from isolated mitochondrial membranes, purified to apparent homogeneity using ion exchange and hydroxylapatite chromatographies. The isolated enzyme catalyzes neither NADH->NADP+ nor NADH->NAD+ transhydrogenations
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1fusion protein using His-tag and on calmodulin-sepharose
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1His-tagged H91E mutant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1Ni-NTA resin column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1phenyl-Sepharose column chromatography and DEAE-Trisacryl M column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1purification of bacterially expressed, recombinant membrane protein fused with calmodulin-binding domains. This method allows isolation of the protein fusions in a single chromatography step using elution with the calcium chelating agent EDTA. Unlike purification of His-tagged proteins on nickel chelate, it is not sensitive to the presence of strong reducing agents (e.g., DTT). The protocol involves disruption of host bacteria by sonication, sedimentation of membranes by differential centrifugation, solubilization of membrane proteins and affinity chromatography on calmodulin-agarose. To achieve maximum purity and yield, the use of a combination of non-ionic and anionic detergents is suggested. Purification takes two working days, with an overnight wash of the column to increase the purity of the product
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1Q-Sepharose column chromatography and Mono Q column chromatography
Display the word mapDisplay the reaction diagram Show all sequences 7.1.1.1Q-Sepharose column chromatography, and butyl Toyopearl column chromatography
Results 1 - 10 of 17 > >>