EC Number   |
|---|
    6.3.3.2 | - |
| 6.3.3.2 | Ni-NTA affinity chromatography and gel filtration |
| 6.3.3.2 | recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis |
| 6.3.3.2 | recombinant His-tagged enzyme from Escherichia coli |
| 6.3.3.2 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography |
| 6.3.3.2 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity |
| 6.3.3.2 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to over 95% homogeneity |
| 6.3.3.2 | recombinant truncation mutant comprising residues 6-202 from Escherichia coli strain BL21(DE3) by affinity chromatography and substrate competition elution |
| 6.3.3.2 | Talon metal affinity column chromatography |
| 6.3.3.2 | Talon metal affinity resin chromatography and Sephadex G-25 gel filtration |
