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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 6.3.2.43Display the reaction diagram Show all sequences 6.3.2.43-
Show all pathways known for 6.3.2.43Display the reaction diagram Show all sequences 6.3.2.43recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-CodonPlus (DE3)-RIL by heat treatment at 80°C for 20 min and nickel affinity chromatography. Recombinant nontagged enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 80°C for 20 min, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultrafiltration, and gel filtration
Show all pathways known for 6.3.2.43Display the reaction diagram Show all sequences 6.3.2.43recombinant enzyme
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