EC Number   |
|---|
   6.2.1.45 | His6-tagged E1 expressed and purified in Saccharomyces cerevisiae MHY-501 cells |
| 6.2.1.45 | Ni-NTA column chromatography |
| 6.2.1.45 | recombinant enzyme, purification on Ni-NTA superflow sepharose and strep-tactin sepharose which is based on UB-UBE1 high-energy thioester bonded intermediate complex |
| 6.2.1.45 | recombinant FLAG-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by affinity chromatography |
| 6.2.1.45 | recombinant GST-tagged enzyme from Hi5 insect cells by glutathione affinity chromatography, tag cleavage by thrombin, and gel filtration |
| 6.2.1.45 | recombinant His-tagged chimeric mutant Aos1-Uba2 SUMO-E1 enzyme mAU from insect cells by nickel affinity chromatography |
| 6.2.1.45 | recombinant His-tagged enzyme domains FCCH, 4HB, SCCH and UFD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, incase of domain UFD purification containing 6 M urea, followed by dialysis, and ultrafiltration |
| 6.2.1.45 | recombinant His-tagged N-terminal enzyme domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, anion exchange chromatography, tag cleavage by 3C protease, another step of nickel affinity chromatography, followed by gel filtration |
| 6.2.1.45 | recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by nickel affinity chromatography |
| 6.2.1.45 | recombinant mRFP-enzyme from Escherichia coli strain DH5alpha by combined anion exchange/affinity chromatography and gel filtration |
