EC Number   |
|---|
    6.2.1.20 | - |
| 6.2.1.20 | a single enzyme protein catalyzes two activities: 2-acylglycerophosphoethanolamine acyltransferase activity and acyl-[acyl-carrier protein] synthetase activity |
| 6.2.1.20 | Ni-NTA column chromatography |
| 6.2.1.20 | partial |
| 6.2.1.20 | partial purification by DEAE-Sepharose chromatography and Sephacryl S-300 gel filtration |
| 6.2.1.20 | recombinant His-tagged enzyme from Escherichia coli |
| 6.2.1.20 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) Rosetta to over 90% purity, purified AasC catalyzed the ATP-dependent ligation of free fatty acids to ACP |
| 6.2.1.20 | recombinant N-terminally His6-tagged enzyme, full-length or lacking the predicted plastidial targeting signal sequence, from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography, gel filtration, and dialysis. Removing the sequence encoding a predicted plastidial targeting peptide increases the expression level significantly, like DELTAaas, DELTAaas:AAE15 secretes large amounts of fatty acids into the culture medium. Expression of AAE15 in the DELTAaas strain does not affect fatty acid profiles and does not have a significant complementation effect |
| 6.2.1.20 | recombinant proteins using His-tag |
