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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7- 11, 156, 510, 516, 517, 520, 521, 525, 651165, 662825, 663256
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7Ni-NTA agarose column chromatography, and gel filtration 728753
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7nickel-chelating Sepharose resin column chromatography 727129
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7partial, from a multienzyme complex containing at least 16 aminoacyl-tRNA synthetase activities 92
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7purified by Ni-NTA chromatography and SDS gel electrophoresis 692360
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7recombinant full-length AlaRS and AlaRS domains from Escherichia coli strain BL21 by anion exchange chromatography followed by hydrophobic interaction and adsorption chromatography, respectively 671126
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 745185
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7recombinant His6-tagged full-length enzyme and C-terminal domain C-Ala from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, followed by tag cleavage through TEV protease, tag and TEV protease are removed by another turn of nickel affinity chromatography, and ultrafiltration and dialysis 744473
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7recombinant His6-tagged wild-type and chimeric fusion enzymes by nickel affinity chromatography and dialysis 745765
Show all pathways known for 6.1.1.7Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.7recombinant PH0574, from Escherichia coli strain BL21 by two steps of anion exchange chromatography, hydroxyapatite chromatography, and gel filtration 677045
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