EC Number |
---|
6.1.1.18 | - |
6.1.1.18 | IgG Sepharose column chromatography and Superdex HiLoad 16/60 gel filtration |
6.1.1.18 | Ni-NTA agarose column chromatography |
6.1.1.18 | partial |
6.1.1.18 | recombinant His-tagged chimeric mutant enzyme from strain BL21(DE3) or the temperature sensitive strain JP1449(DE3) by nickel affinity chromatography |
6.1.1.18 | recombinant His-tagged GlnRS mutants from strain BL21-DE3 by nickel affinity chromatography |
6.1.1.18 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography and dialysis |
6.1.1.18 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 |
6.1.1.18 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3)pLysS by nickel affinity chromatography and gel filtration |
6.1.1.18 | recombinant His6-tagged wild-type full-length enzyme and truncated mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration, optimization of purification conditions |