EC Number   |
|---|
   5.6.1.4 | - |
| 5.6.1.4 | 6His-tagged full-length Klp2. a Klp2 protein lacking the motor domain (Klp2-tail) and a Klp2 protein containing only the motor domain and a short coiled coil to mediate dimerization (Klp2-motor) are purified by ammonium sulfate precipitation and HisTrapHP column chromatography |
| 5.6.1.4 | fusion protein GST-Kar3 |
| 5.6.1.4 | glutathione Sepharose column chromatography |
| 5.6.1.4 | GST-bind resin chromatography and NAP-5 gel filtration |
| 5.6.1.4 | His6Kar3/Cik1-S tag protein |
| 5.6.1.4 | immobilized metal ion affinity chromatography (Ni2+) |
| 5.6.1.4 | immobilized metal ion affinity chromatography (Ni2+), gel filtration |
| 5.6.1.4 | ion exchange chromatography (SP Sepharose) and ion exchange chromatography (MonoQ) or gel filtration |
| 5.6.1.4 | MC1 and MC6 |
