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EC Number
Purification (Commentary)
Reference
5.4.99.18
-
1268
,
663416
5.4.99.18
recombinant enzyme to over 85% purity
747253
5.4.99.18
recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration
726578
5.4.99.18
recombinant N-terminally His-tagged enzyme by nickel affinity chromatography, and tag cleavage by thrombin, followed by another step of nickel affinity chromatography, benzamidine affinity chromatography, and gel filtration
747257
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