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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18-
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18recombinant enzyme to over 85% purity
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18recombinant His-tagged enzyme from Escherichia coli strain BL21 Star (DE3) by nickel affinity chromatography, tag cleavage by TEV protease, and gel filtration
Show all pathways known for 5.4.99.18Display the word mapDisplay the reaction diagram Show all sequences 5.4.99.18recombinant N-terminally His-tagged enzyme by nickel affinity chromatography, and tag cleavage by thrombin, followed by another step of nickel affinity chromatography, benzamidine affinity chromatography, and gel filtration
Results 1 - 4 of 4
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