EC Number |
---|
5.4.99.13 | Ni-NTA-Sepharose column chromatography, Source 15Q column chromatography, and Superdex 200 gel filtration |
5.4.99.13 | partial, using ammonium sulfate precipitation, and DEAE-Sepharose column chromatography |
5.4.99.13 | recombinant His-MBP-tagged wild-type enzyme and His6-SUMO-tagged mutant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, tag cleavage by TEV protease, dialysis, amylose affinity chromatography (only wild-type), and gel filtration |
5.4.99.13 | recombinant IcmB is purified using Q-Sepharose, and two Mono Q column chromatography |
5.4.99.13 | recombinant N-terminally His-tagged |
5.4.99.13 | using ammonium sulfate precipitation, DEAE, Q-sepharose, superdex, gel electrophoresis, and B12 affinity column chromatography |