EC Number |
---|
5.4.99.1 | - |
5.4.99.1 | after individual expression of SanU and SanV in Escherichia coli |
5.4.99.1 | cloning and overexpression in Escherichia coli allows component E to be obtained in homogeneous form, free of inhibiting cobamides and traces of component S |
5.4.99.1 | fusion protein in which the cobalamin-binding subunit is linked to the catalytic subunit |
5.4.99.1 | purification of component E and component S |
5.4.99.1 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) pLysS by nickel affinity chromatograpyh and ultrafiltration, recombinant expression in Escherichia coli strain BW25113, coexpression with Clostridium tetanomorphum 3-methylaspartate ammonia lyase |