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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 5.1.99.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.99.7native enzyme 954fold by ammonium sulfate fractionation, Cibacron blue 3GA affinity chromatography, hydrophobic interaction chromatography, methotrexate affinity chromatography, and ge filtration, to homogeneity 734122
Show all pathways known for 5.1.99.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.99.7recombinant enzyme 733859
Show all pathways known for 5.1.99.7Display the word mapDisplay the reaction diagram Show all sequences 5.1.99.7recombinant enzyme 2.7fold from Escherichia coli strain M15 by anion exchange chromatography, ultrafiltration, heat treatment at 80°C for 4 min, and gel filtration 5109
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