5.1.2.1 | recombinant StrepII-tagged LarA from Lactobacillus lactis by affinity chromatography, gel filtration, and ultrafiltration, to homogeneity. Purification of LarA and LarE can readily be achieved from the strain expressing the entire operon, whereas tagged LarB and LarC can only be purified when the corresponding genes are individually subcloned |