EC Number |
---|
5.1.1.15 | - |
5.1.1.15 | ACL racemase is purified from Escherichia coli JM109/pACL60 |
5.1.1.15 | native enzyme by several steps of ion exchange and hydrophobic interaction chromatography, dialysis, ultrafiltration, and gel filtration, recombinant His-tagged enzyme 31.9fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity |
5.1.1.15 | partial |
5.1.1.15 | recombinant |
5.1.1.15 | recombinant His-tagged enzyme 129fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
5.1.1.15 | recombinant His-tagged enzyme 27.9fold from Escherichia coli strain BL21(DE3) by heat treatment, nickel affinity chromatography, and dialysis |
5.1.1.15 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and dialysis |
5.1.1.15 | using Ni Sepharose highperformance (Amersham) and anion-exchange chromatography (HiTrapQFF) |