EC Number   |
|---|
    4.3.2.2 | - |
| 4.3.2.2 | Ni-affinity chromatography |
| 4.3.2.2 | Ni-NTA agarose column chromatography |
| 4.3.2.2 | normal and mutant enzyme S413P |
| 4.3.2.2 | Q-Sepharose column chromatography and gel filtration |
| 4.3.2.2 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography |
| 4.3.2.2 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta 2(DE3)pLysS by nickel affinity chromatography, dialysis, cleavage of the His-tag through thrombin, and gel filtration, to homogeneity |
| 4.3.2.2 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatography |
| 4.3.2.2 | using a Ni-NTA column |
| 4.3.2.2 | using a Ni-NTA column, the 6-histidine tag is removed by cleavage with thrombin, separation of thrombin and histidine-tag from the enzyme is achieved by using a Sephadex G-100 column |
