EC Number |
---|
4.3.1.17 | - |
4.3.1.17 | anaerobic purification of His-tagged enzyme, purified protein is brown in colour |
4.3.1.17 | both isoforms A and B |
4.3.1.17 | cSDH is purified using a Sephacryl S-200 and a Ni2+-NAT column |
4.3.1.17 | expression in Escherichia coli with N-terminal His-tag, purification protocol from inclusion bodies |
4.3.1.17 | immobilized metal ion affinity chromatography (Ni2+) |
4.3.1.17 | partial |
4.3.1.17 | partially purified |
4.3.1.17 | proteins of crude extracts are fractionated using a DEAE-Sephadex A-25 column |
4.3.1.17 | recombinant cancer cell isozyme cSDH to homogeneity by ammonium sulfate fractionation, dialysis, anion exchange and nickel affinity chromatography |