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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.16-
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.16after centrifugation of cultivated cell culture with the recombinant enzyme, resulting supernatant is applied to a Ni-sepharose column connected to a fast protein liquid chromatography system. The column is equilibrated with buffer supplemented with 20 mM imidazole. The enzyme is eluted with imidazole gradient.
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.16recombinant His-tagged enzyme
Results 1 - 3 of 3