EC Number   |
|---|
   4.3.1.1 | - |
| 4.3.1.1 | After overexpression of the aspA gene in Escherichia coli BL21, the gene product is purified to homogeneity by ion-exchange and hydrophobic interaction chromatography |
| 4.3.1.1 | by using a combination of diethylaminoethyl cellulose, Red A-agarose,and Sepharose 6B chromatography |
| 4.3.1.1 | immobilized metal ion affinity chromatography (Ni2+) |
| 4.3.1.1 | method |
| 4.3.1.1 | overview |
| 4.3.1.1 | partial |
| 4.3.1.1 | partially purified by heat precipitation and saturation with ammonium sulfate |
| 4.3.1.1 | recombinant enzyme containing a C-terminal His6 tag is purified by one-step affinity purification |
| 4.3.1.1 | recombinant enzyme expressed in Escherichia coli |
