EC Number   |
Reference |
|---|
   4.2.2.7 | - |
5972, 5973, 5976, 5978, 5982, 5986, 663480, 665440, 665714, 681056 |
| 4.2.2.7 | ammonium sulfate precipitation followed by DEAE-Sephacel and Sephacryl S-200 column chromatography |
650632 |
| 4.2.2.7 | by several chromatographic steps, including QAE-cellulose, DEAE-cellulose, Sephadex and hydroxyapatite |
651770 |
| 4.2.2.7 | enhancement of enzyme production by 92% is achieved by manipulation of the fermentation conditions (pH: 6.5, inoculum size of 0.5-1.0%, incubation temperature 30°C) and medium ingredients (replacement of dextrose by mannitol, soy peptone by chitin and ammonium sulfate by ammonium nitrate and supplementation of pyridoxine hydrochloride and glutaric acid) |
693981 |
| 4.2.2.7 | enzyme expressed in Escherichia coli as a fusion protein with GST at the N-terminus, one-step affinity purification |
677819 |
| 4.2.2.7 | immobilization |
5981, 5982 |
| 4.2.2.7 | immobilized metal ion affinity chromatography, ion exchange chromatography, gel filtration |
704591 |
| 4.2.2.7 | ion exchange chromatography |
704233 |
| 4.2.2.7 | ion-exchange chromatography (SP-Sepharose), hydroxylapatite chromatography, mutant proteins by immobilized metal-chelate chromatography, His-tag is removed, further purification by ion-exchange chromatography |
704673 |
| 4.2.2.7 | large scale |
5979, 5986 |
