EC Number   |
|---|
    4.2.1.75 | - |
| 4.2.1.75 | expression in Escherichia coli |
| 4.2.1.75 | from overproducing strain |
| 4.2.1.75 | Ni-NTA column chromatography and Superdex 75 gel filtration |
| 4.2.1.75 | partial |
| 4.2.1.75 | recombinant |
| 4.2.1.75 | recombinant His-tagged enzyme from Escherichia coli |
| 4.2.1.75 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, and gel filtration to homogeneity |
| 4.2.1.75 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the tag is cleaved off by TEV, followed by gel filtration |
| 4.2.1.75 | recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration |
