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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47-
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47by ultrasonication, centrifugation, on Ni2+-nitrilotriacetic acid resin and by anion exchange chromatography, to greater than 95% purity
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47GSH-Sepharose 4B column chromatography
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47Hi-Trap chelating column chromatography
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47only isoenzyme L-GMD and isoenzyme M-GMD are soluble, isoenzyme S-GMD is inactive and forms a precipitate
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47recombinant His-tagged GMD from Escherichia coli by nickel affinity chromatography
Show all pathways known for 4.2.1.47Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.47to near homogeneity by nickel ion affinity chromatography
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