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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115recombinant GST-tagged enzyme from Escherichia coli strain BL-21 GOLD by glutathione affinity chromatography, proteolytic removal of the GST-tag, and ultrafiltration, recombinant His6-tagged enzyme from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography, proteolytic removal of the His-tag, another step of nickel affinity chromatography, and desalting gel filtration, thereby the recombinant protein corresponds to the native enzyme in which the initial methionine is replaced by GPGS sequence resulting from the cleavage site
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115recombinant His-tagged FlaA1 9.9fold from Escherichia coli strain BL21(DE3) to homogeneity by nickel chelation and cation exchange chromatography
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)
Display the word mapDisplay the reaction diagram Show all sequences 4.2.1.115recombinant N-terminally His-tagged and C-terminally His-tagged WbpMs from Escherichia coli by selective solubilization from the inner membrane by lauryl sarcosine and nickel chelation chromatography
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