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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number Purification (Commentary) Reference
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38DEAE-Sephacel, phenyl-Sepharose column, Superose 6 gel filtration column, Mono Q, Superose 12 column, yield 400-800fold 644227
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38fractionation with ammonium sulfate pH 7.5, DEAE-Sephacel column, butyl-Sepharose 4B column, Gigapite column 644232
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38fractionation with ammonium sulfate, DEAE-Toyopearl column, butyl-Toyopearl column and Mono Q column 644231
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38nickel affinity column chromatography 705561
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38reactive yellow 3-agarose column, Mono Q HR 5/5 FPLC column, Superose 12HR 10/30 FPLC column, Mono Q HR 5/20 FPLC column, Aquapore RP-300 C8 HPLC column: 4100fold to near homogeneity 644229
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) and B834(DE3), respectively, by nickel affinity chromatography 727950
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38recombinant His6-tagged enzyme from Escherichia coli by metal affinity chromatography 726913
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) GOLD by nickel affinity chromatography, cleavage of the tag by TEV protease, and gel filtration 728557
Show all pathways known for 4.1.3.38Display the reaction diagram Show all sequences 4.1.3.38Resource ISO column chromatography, ammonium sulfate precipitation, Resource Q column chromatography, and Superdex 75 gel filtration 701506
Results 1 - 9 of 9