EC Number   |
|---|
    4.1.1.98 | Ni-NTA agarose bead chromatography and Sephacryl S200 gel filtration |
| 4.1.1.98 | Ni-NTA resin column chromatography and Superdex 200 gel filtration |
| 4.1.1.98 | Ni-NTA resin column chromatography and Superose 12 gel filtration |
| 4.1.1.98 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration |
| 4.1.1.98 | UbiD cannot be isolated in an active holoenzyme form. Formation of holoUbiD requires reconstitution in vitro of the apoUbiD with reduced prenylated FMN. The apoenzyme can be readily reconstituted and activated, but in vitro oxidation to the mature prenylated FMN cofactor stalls at formation of a radical prenylated FMN species in holoUbiD |
