EC Number   |
|---|
    4.1.1.90 | - |
| 4.1.1.90 | affinity chromatography |
| 4.1.1.90 | degree of purification is about 7000fold with reference to ammonium sulfate-fractionated microsomal protein from liver. Purification of carboxylase, solubilized microsomes: 281000 cpm/mg/h, flow-through of Affi-FIXQ/S: 200000 cpm/mg/h, bound to Affi-FIXQ/S: 140000000 cpm/mg/h, affinity-purified carboxylase: 1930000000 cpm/mg/h |
| 4.1.1.90 | difficult, different methods shown |
| 4.1.1.90 | inactivated His6-carboxylase-Ac-FLEEL purified under denaturing conditions by Ni-chelation chromatography followed by preparative polyacrylamide gel electrophoresis |
| 4.1.1.90 | partial purification of the enzyme by antibody affinity techniques. Purified 500fold by adsorption to an antiprothrombin column and elution with a dodeca peptide which competes with a prothrombin precursor enzyme recognition site. The purified enzyme is devoid of bound precursors, and has the same ratio of vitamin K epoxidase activity to carboxylase activity as the crude microsomal preparation |
