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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 4.1.1.111Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.111Ni-NTA column chromatography and NAP-25 Sephadex gel filtration 746941
Show all pathways known for 4.1.1.111Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.111Ni-NTA column chromatography and Superdex 75 gel filtration 748393
Show all pathways known for 4.1.1.111Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.111Ni-Sepharose column chromatography and Superdex 200 gel filtration 748682
Show all pathways known for 4.1.1.111Display the word mapDisplay the reaction diagram Show all sequences 4.1.1.111proteins ahbA and ahbB are cloned into distinct plasmids and expressed separately in Escherichia coli. When expressed individually the encoded proteins are highly unstable and can only be purified at very low concentrations due to their tendency to precipitate 726843
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