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Search Purification (Commentary)

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Results 1 - 7 of 7
EC Number Purification (Commentary) Reference
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2- 648277, 648279, 648289, 648292, 648298, 648299, 648300, 648301, 648302, 648303, 648304, 648305, 648307, 648309, 723926, 756964, 757261
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2expression in Escherichia coli 667850
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration 695317
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2Ni-NTA column chromatography and gel filtration 697836
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity 735151
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography and gel filtration 733826
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production 723963
Results 1 - 7 of 7