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Search Purification (Commentary)

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Results 1 - 7 of 7
EC Number Purification (Commentary)
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2-
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2expression in Escherichia coli
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2Ni-NTA column chromatography and gel filtration
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography and gel filtration
Show all pathways known for 3.8.1.2Display the word mapDisplay the reaction diagram Show all sequences 3.8.1.2two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production
Results 1 - 7 of 7