EC Number   |
|---|
   3.6.5.1 | - |
| 3.6.5.1 | Go |
| 3.6.5.1 | purified from Sulfolobus solfataricus |
| 3.6.5.1 | recombinant His6-tagged truncation enzyme mutants GalphaoDELTA21 and GalphaoDELTA109 from Escherichia coli strain M15 by nickel affinity chromatography, for the first followed by anion exchange chromatography and gel filtration |
| 3.6.5.1 | the alpha subunit of Gq family G proteins, GL1alpha(G14alpha), GL2alpha(G11alpha) and Gqalpha are expressed with G proteins beta1 and gamma2 subunits in insect cells using a baculovirus system. The trimeric forms of G proteins, GL1(GL1alphabetagamma), GL2(GL2alphabetagamma) and Gq(Gqalphabetagamma) are solubilized and purified |
| 3.6.5.1 | using a Ni-NTA column |
| 3.6.5.1 | using Ni-NTA His-binding affinity chromatography and a Mono Q column |
