EC Number |
---|
3.6.4.B8 | - |
3.6.4.B8 | recombinant clamp loader complex subunits delta, gamma, delta', chi, and psi |
3.6.4.B8 | recombinant His-tagged mini-delta from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the His tag by thrombin, dialysis, and gel filtration |
3.6.4.B8 | recombinant replication factor C subunits RFC2, 3, 4, and 5 with mutated truncated subunit RFC1 |
3.6.4.B8 | the two SsoRFC polypeptides are coexpressed in Escherichia coli and purified as a complex. The small subunit is individually expressed in Escherichia coli and purified |