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Search Purification (Commentary)

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Results 1 - 10 of 10
EC Number Purification (Commentary) Reference
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6- 648215, 648216, 648217, 648218, 648219, 648220, 648221, 648223, 648224, 648226, 648228, 648236
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6ammonium sulfate precipitation, Resource Q column chromatography, and Ni2+-IDA agarose column chromatography 734837
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6N-6-aminohexanoyl-glucosamine 6-phosphate agarose column chromatography 735143
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6native enzyme 1390fold from kidney cortex by affinity chromatography on N-epsilon-amino-n-hexanoyl-D-glucosamine-6-phosphate agarose and on phosphocellulose, the latter behaving as an active site affinity resin 671576
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6native enzyme by ammonium sulfate fractionation, anion exchange chromatography, and affinity chromatography on a N-aminohexyl-glucosamine-6-phosphate resin folowed by dialysis and ultrafiltration 684638
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6recombinant enzyme 648241
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6recombinant enzyme NagB from Escherichia coli strain BL21(DE3) by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration, to near homogeneity 753578
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity in two steps by nickel affinity chromatography and gel filtration 684212
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6recombinant isozyme GNPDA1 from Escherichia coli strain IBPC 590 by single purification step by means of allosteric-site affinity chromatography on N-6-aminohexanoyl-GlcN6P agarose 718986
Show all pathways known for 3.5.99.6Display the word mapDisplay the reaction diagram Show all sequences 3.5.99.6wild-type enzyme and two mutants 648237
Results 1 - 10 of 10