EC Number   |
|---|
   3.5.5.5 | - |
| 3.5.5.5 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration |
| 3.5.5.5 | cells of bacteria culture are disrupted and centrifugation and the enzyme in the soluble fraction is purified using Ni-NTA affinity chromatography |
| 3.5.5.5 | DEAE Sepharose Fast Flow column chromatography and Sephacryl S-300 gel filtration |
| 3.5.5.5 | Ni-NTA column chromatography |
| 3.5.5.5 | partial, 259fold |
| 3.5.5.5 | recombinant enzyme 2.4fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity |
| 3.5.5.5 | recombinant enzyme 3fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity |
| 3.5.5.5 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
