EC Number |
---|
3.5.4.10 | - |
3.5.4.10 | 154-fold |
3.5.4.10 | 780fold to apparent homogeneity |
3.5.4.10 | expressed as glutathione-S-transferase fusion protein in Escherichia coli mutants |
3.5.4.10 | from liver homogenate |
3.5.4.10 | near to homogeneity from Escherichia coli expression mutants |
3.5.4.10 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity and anion exchange chromatography |
3.5.4.10 | recombinant His-tagged enzyme from Escherichia coli strain BL21 by cobalt affinity chromatography, elution with imidazole, to homogeneity |
3.5.4.10 | soluble recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 by heat treatment for 15 min at 70°C, and anion exchange chromatography, to homogeneity |