EC Number |
---|
3.5.3.12 | - |
3.5.3.12 | 2470fold |
3.5.3.12 | enzyme purified from shoots grown in the dark by DE52 column chromatography, Sephadex G-100 column chromatography and agmatine-epoxy-Sepharose 6B column chromatography |
3.5.3.12 | HisTrap Ni-affinity column chromatography and Superdex 200 HR gel filtration |
3.5.3.12 | nickel-chelated agarose gel column chromatography |
3.5.3.12 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography |
3.5.3.12 | strain PAO4495, 21fold purified to near homogeneity, gel filtration |
3.5.3.12 | the protein is recombinantly expressed, lysed by sonication, and then purified by a combination of anion exchange, heparin affinity, and gel filtration |