EC Number   |
|---|
    3.5.2.5 | - |
| 3.5.2.5 | DEAE-Sephacel column chromatography, MonoQ HR column chromatography, and Sephacryl S-300 gel filtration |
| 3.5.2.5 | native enzyme from seeds |
| 3.5.2.5 | native isozymes 1 and 2 from developing fruits 150fold and 286fold, respectively, to homogeneity involving heat treatment at 55°C for 20 min, anion exchange chromatography, gel filtration, and another step of anion exchange chromatography |
| 3.5.2.5 | recombinant by gel filtration and affinity chromatography to near homogeneity |
| 3.5.2.5 | recombinant enzyme |
| 3.5.2.5 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
| 3.5.2.5 | recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, ultrafiltration, and dialysis |
| 3.5.2.5 | recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, gel fltration, and ultrafiltration |
| 3.5.2.5 | Sephacryl S-200 column gel filtration chromatography at 0-4°C |
