EC Number |
---|
3.5.1.97 | - |
3.5.1.97 | a two-step purification protocol, anion exchange chromatography and hydrophobic interaction chromatography |
3.5.1.97 | gene macQ, recombinant His-tagged enzyme from Escherichia coli strain Origami 2(DE3) by nickel affinity chromatography |
3.5.1.97 | native enzyme 155.8fold from strain VM4 by ammonium sulfate fractionation, dialysis, anion exchange chromatography, ultrafiltration, and gel filtration |
3.5.1.97 | Ni-NTA agarose column chromatography |
3.5.1.97 | recombinant enzyme from Escherichia coli strain DH10B by several chromatographic steps |
3.5.1.97 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |