EC Number   |
|---|
   3.5.1.49 | expressed in the soluble form by Escherichia coli XL1 Blue MRF cells (5mg fusion protein per liter culture), purified on a Glutathione Sepharose 4B Resin column, cleaved with thrombin |
| 3.5.1.49 | glutatione Sepharose column chromatography, DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography |
| 3.5.1.49 | MagneHis protein purification system |
| 3.5.1.49 | Ni-NTA His-binding affinity chromatography, gel filtration on a Sephacryl S-200 column |
| 3.5.1.49 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration |
| 3.5.1.49 | recombinant protein |
| 3.5.1.49 | Talon metal-affinity resin column chromatography and Superdex 200 gel filtration |
| 3.5.1.49 | to homogeneity |
