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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.4829fold, chromatography techniques
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.48partial
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.48recombinant TEV-cleavable N-terminal His-MBP-tagged enzyme from Escherichia coli strain BL21(DE3) by amylose affinity chromatography and anion exchange chromatography, the MBP-tag is not cleaved off
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.48recombinant TEV-cleavable N-terminal His-MBP-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by amylose affinity chromatography, TEV protease is used to remove the His-MBP-tag, followed by anion exchange chromatography and gel filtration
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