EC Number   |
|---|
    3.5.1.42 | - |
| 3.5.1.42 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, Reactive Red 120-agarose column chromatography, Resource Q column chromatography, and Superose 12 gel filtration |
| 3.5.1.42 | near homogeneity, chromatography techniques |
| 3.5.1.42 | partial |
| 3.5.1.42 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography and gel filtration |
| 3.5.1.42 | recombinant His6-tagged enzyme from Escherichia coli strain T7 by nickel affinity chromatgraphy, cleavage of the tag by thrombin, anion exchange chromatography, and gel filtration |
| 3.5.1.42 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nicke affinity chromatography |
| 3.5.1.42 | to homogeneity, chromatography techniques |
