EC Number   |
|---|
   3.4.24.28 | - |
| 3.4.24.28 | after expression in Escherichia coli with N-terminal His-tag |
| 3.4.24.28 | extracellular enzyme from culture medium by anion exchange chromatography |
| 3.4.24.28 | extracellular enzyme from culture medium by several chromatographic steps including one affinity based step |
| 3.4.24.28 | native enzyme 13.1fold by ethanol precipitation, gel filtration, and anion exchange chromatography |
| 3.4.24.28 | native extracellular enzyme 5.6fold from culture supernatant by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, and gel filtration |
| 3.4.24.28 | recombinant enzyme |
| 3.4.24.28 | recombinant enzyme from Bacillus subtilis culture medium by ammonium sulfate fractionation and hydrophobic interaction chromatography to homogeneity |
| 3.4.24.28 | recombinant enzyme from Escherichia coli strain BL21(DE3) |
| 3.4.24.28 | wild-type and mutant enzymes |
